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非细胞体系的 DNA 甲基化测序
DNA甲基化

优化从液体活检样本中分离 cfDNA

非细胞体系的 DNA 甲基化测序

细胞中游离 DNA 的甲基化研究

亚硫酸氢盐转化DNA文库的制备和下一代测序技术的进步,使得能够从血浆或血清样本中存在的少量输入DNA中测量甲基化水平。具体的 基因组 可以使用亚硫酸氢盐测序通过选择特定的 DNA 序列来监测感兴趣的区域,例如 CpG 岛。研究人员可以使用甲基化测序来了解构成复杂疾病的机制。

方达 已开发并验证了通过测序进行靶向和全局甲基化分析的协议,并优化了从液体活检样本中分离 cfDNA 以最大限度地减少该服务的样本输入要求。

  • 体液分析
  • 测序
  • 生物标志物发现
  • 伴随诊断
  • 预测建模

适用于 cfDNA 亚硫酸氢盐测序的样品类型

样本类型包括全血、血清和血浆。 方达提供基于血液的生物流体样本的 cfDNA 分离服务。我们是生物安全 2 级 (BSL-2) 实验室,可以接受含有与人类疾病相关的试剂的样本。

样品类型 最低输入要求 样品提交清单
游离DNA 10 ng cfDNA
体液 4 ml 体液

 

gDNA 读取摘要

  • 用基因组坐标、每个样本的计数和注释检测序列读数。

 

标准 cfDNA 亚硫酸氢盐测序报告

  • 识别甲基化位点,显示每个位点的甲基化调用和甲基化百分比。
  • 统计分析结果显示,甲基化位点在组间的甲基化水平、最近基因的注释以及甲基化位点与基因的相对位置上存在显著差异。
  • 富含CpG的基因组区域的图谱,表明差异甲基化区域在染色体上的分布。
  • CpG 覆盖率和 CpG 甲基化水平的分布直方图。

DNA 和人 - 无细胞 DNA 甲基化测序

额外的原始数据

  • 压缩的 FASTQ 文件
  • 映射到基因组的原始独特序列(FASTA 格式)
  • 原始和标准化读取计数,以及读取映射到每个查询的每个数据库的每个 gDNA

 

 

方达致力于提供严格的科学专业知识,以确保最高质量和合规性。我们的 药物发现 实验室服务团队拥有在药物开发各个阶段与您合作的专业知识。

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